An integrated, easy-to-use, and cost-effective technology platform for the rapid handling and growth of large numbers of microbial strains, clonal libraries, mutant banks, and cell lines in 24 or 96-well microtiter plates.

The system enables a single person to grow and test thousands of strains simultaneously with a minimum of repetitive handlings: all handling is performed in parallel for sets of 24 or 96 strains.

More efficient use of your current orbital shaker: time savings up to a factor 10 possible.

Same quality of growth as in Erlenmeyer flasks in terms of oxygen transfer rates and reproducibility: See it for yourself; view the technical data, photos and movies showing the hydrodynamics inside the wells during orbital shaking.

MicroFlask by Duetz is used with 

• 24 square deepwell plates (2.5 - 4 ml cultures)
• 96 square deepwell plates (0.5 - 1 ml cultures)
• 24 round lowwell plates (0.75-1.5 ml cultures)
• 96 round lowwell plates (0.1 - 0.2 ml cultures)

Applications

• Discovery: high-throughput screening for new enzyme activities or formation of secondary metabolites (heterogeneous culture collections)
• Screening and distribution of clone libraries in E. coli or yeasts
• High-throughput screening for high-activity or high-productivity mutants
• Comparative studies e.g. clinical isolates
• Growth medium optimization studies

Micro-Flask Main components

    Cassettes, Sandwich covers , clamps.

Options The cryo-replicator

Sandwich covers convert all wells of microtiter plates into individual "mini-reactors" suitable for high-speed orbital shaking, at high oxygen transfer rates, and without well-to-well variations.

All sandwich covers consist of a stainless steel cover, 0,2µm filter and microfiber inlays and a flexible silicone layer for sealing.

The MicroFlask system makes your existing shaker more eficcient; three 24-lowwell plates or four 96-lowwell plates are stackable per clamping position. 

Can be used with the PreSens SDR SensorDish Reader®. The SDR® is a small 24-channel reader for non-invasive detection of oxygen and pH in lowwell plates (SensorDishes). SensorDishes for oxygen (OxoDish®) and pH (HydroDish®) are available in the 24-well and 6-well format. The SensorDish Reader®can be used in incubators and on shakers and is thus the ideal tool for monitoring pH or DO for cultivations in combination with the MicroFlask system.