Cell Disruption

Constant Cell Disruption Systems are based on the use of high pressure to force a sample through a small fixed orifice at high speed under controlled, contained and repeatable conditions.

1. As the high pressure piston descends, sample is introduced into the high pressure cylinder.

2. The high pressure piston then accelerates the sample through the fixed orifice at high velocity. The controlled acceleration causes the cells to rupture.

3. When the jet containing disrupted cells hits the target, it is spread radially across the cooled heat exchange surface. Product then flows from the disruption chamber for collection. The hydraulics are recharged and the cycle continues.

Reproducible Results
Constant Cell Disruption Systems give reproducible and superior results because control of the disruption pressure is achieved by the hydraulic control system. The disruption pressure cycle is shown in the adjacent graph. This has been created by analysing and recording the hydraulic pressure in the operating cylinder, which is related to the disruption pressure by the ratio of the piston areas.

Your Application and Cell Success

Constant Cell Disrupter Systems are extremely versatile and have been used for the following range of applications:

cell disruption,protein&DNA & DNA extraction, enzyme release,DNA manipulation,selective breakage, single cell isolation, tissue disruption.

Examples of cells successfully processed by Constant Systems Limited are too numerous to mention them all, but here follows a few examples.